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The buffer was 20 mM imidazole pH seven, with the addition of 1 mM DTT and 1 mM EDTA when T6P synthase was assayed. The extract was centrifuged in the chilly for fifteen min at 13000 rpm in an Eppendorf table best centrifuge and the supernatant utilised for determination of enzyme pursuits. T6P synthase activity was identified by a two action technique in a mixture of 50 mM imidazole pH 7, .1 M KC

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