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Whereas DmPI31D211K and DmPI31P243A mutants retained their ability to be able to situation dTNKS, DmPI31L210A and DmPI31F241A were unable to variety a fancy along with dTNKS (Figure?1G). In addition, removal in the HbYX pattern throughout DmPI31 in addition abrogated your discussion among DmPI31 along with dTNKS (Figure?1G, lane 7). This means that in which dTNKS employs their ANK area to be able

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