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For pre-treatment with cyclic nucleotides, appropriate chemical (adenosine 3��5��-cyclic monophosphate sodium salt monohydrate (cAMP; 10?��M); guanosine 3��5��-cyclic monophosphate sodium salt (cGMP; 10?��M); or the cell permeant analogues 8-Br-cAMP (10?��M) and 8-Br-cGMP (10?��M)) was added to the Petri dish for either 1 or 24?h. Net fluxes #links# of K+ and Ca2+ were measured using non-invasive

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