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Alanine replacing mutants in the PL displayed many different phenotypes throughout dsRNA functionality assays (Fig. 5B). To realize understanding of your step(utes) of dsRNA synthesis during which PL strains meditated their own phenotypic consequences, sodium awareness assays ended up done. From the assays, VP1 PL mutants had been pre-incubated along with pGpG ahead of the inclusion of NTPs (Fig.

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