The walls ended up blotted with the right after antibodies: anti-phospho-PKA substrates (Mobile Signaling #9624), anti- phospho-PKC substrates (Mobile Signaling #2261) anti-ERK as well as anti-phospho-ERK, anti-MEK1/2 as well as anti-phospho-MEK1/2 (all from Cell Signaling). Chemiluminescent diagnosis was executed utilizing supplementary antibodies combined to HRP and also ECL or even ECL?+?substr


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