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Mouse ovary-derived KK1 granulosa mobile or portable line was maintained inside DMEM formulated using antibiotics and solution (10% FBS, Sigma). Atomic extracts were geared up via KK1 mobile collection using the NE-PER Fischer and also Cytoplasmic Elimination reagents (Thermo Technological). Your series with the Runx-1 oligonucleotides specified on the Runx-1 mouse button Genetics along with were

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