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110 �� 6% for rAAV.LacZ, each compared with the respective level at day 7, p = NS), but it was greatly improved when rAAV.PDGF-B was coapplied (189 �� 14%, p < 0.05 vs. both, rAAV.VEGF-A and rAAV.LacZ) (Fig. 2C). Thus, only the combination of VEGF-A and PDGF-B induced both microvascular and macrovascular growth (i.e., generation of pericyte-covered capillaries and an increase in collaterals, with
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Temperature equilibration took area in significantly less than five minutes. In two consecutive PCR reactions a I-SceI restriction web site and a deletion of 658 bp ended up created. Primers 1001 and 1002 with complementary finishes including the recognition web site of meganuclease I-SceI, a thousand and 1003 had been used. With pCLF3 as template and the mentioned primers two fragments correspond