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MiRNA appearance users which are certain to the examined developing stages ended up examined up coming. For this purpose, all of us proceeded #links# analogously towards the identification of tissue-specific miRNAs (Fig. Several). All of us discovered that miR-199b, miR-25, miR-27b as well as miR-200b are usually very abundant in just about all E12 along with E13 libraries (Fig. 5A along with B, c
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, 2006). Mutations to the L protein of TMEV, specifically disruptions made to the zinc-finger domain of this protein, also fail to facilitate the relocalization of endogenous nuclear proteins to the cytoplasm that are relocalized during infection with wild type TMEV (Delhaye et al., 2004). Protease inhibitors fail to suppress the cytoplasmic redistribution of stably expressed EGFP-NLS fusion prote
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